The University of Arizona

Announcements

Arizona Research Laboratories (ARL) and the Materials Science and Engineering Department (MSE) have collaborated to expand the University's spectroscopy and imaging capabilities. All services are open to any investigator in academia, public and private institutions and industry.

more

 

Access Our Services

Please read our policy statement then follow the link to the registration form

 

Resources

ACCESS REQUEST FORM

 

EQUIPMENT SCHEDULER

****************

Other Resource Links

 

Within USIF

USIF Workshops

USIF Outreach

 

BIOTECH Imaging Technologies

NMR/MRI

Flow Cytometry

 

Other University of Arizona Imaging Facilities

 

The University of Arizona

Arizona Research Laboratories

Division of Biotechnology

Materials Science and Engineering

Arizona Imaging and Microanalysis Society

more

University Spectroscopy and Imaging Facilities

Clinical Imaging - Electron Microscopy

USIF - AHSC Imaging

Laser Scanning Confocal Microscopy

This resource is housed at the AHSC Facility located in LSN410. We offer three types of service for Confocal Microscopy.

All services begin with an initial meeting with the USIF - AHSC Imaging Confocal Microscopy Manager. Please contact Doug Cromey 520-626-2824, to discuss your microscopy needs.

Assisted Use - You may drop your samples off to the facility and we will scan and acquire the images for you.

Unassisted Use - After completing the required training session, you may operate the microscope on your own. The training includes the successful completion of the Laser Radiation Protection Course (through the Radiation Control Office).

Training - we will provide hands-on training to effectively operate the microscope. This three session (3 hours each) training program is designed to help you optimize the system for your type of samples. This training is required to take advantage of the Unassisted Scanning service. Laser Radiation Protection Course certification requirement is part of the training.

_______________________________________

Rates

Effective: 12/01/2007

2-photon laser $35.00/Hr.
2-photon laser, Training $43.00/Hr.
Assisted Confocal $71.00/Hr.
Unassisted Confocal $28.00/Hr.
Confocal Training $43.00/Hr.

External customers: External rates plus an additional 8.7% Administrative Service Charge will be assessed.

Please take the time to view the Confocal Microscopy Service Policies.

Schedule the Zeiss Confocal Microscope SCHEDULE
the Zeiss Confocal Microscope

About the Zeiss Confocal Microscope At the AHSC Facility

Pic 2

Zeiss LSM 510 META NLO

(2000's) Upright Axioplan 2 imaging microscope with a motorized XY scanning stage and a META scanning module capable of analyzing spectral signals from UV to NIR.  Objectives include 10X dry, 25X multi-immersion, 40X dipping lens for visualizing samples in culture media, 40X and 100X oil immersion, and a 63X water immersion objective.  Available excitation laser lines include: 457, 477, 488, 514, 543, and 633 nm.  The Coherent MIRA900 2-photon laser is tunable from 700-950 nm.

Confocal Microscopy Policies

The Confocal Imaging microscope is available to all researchers in the AZ state university system as well as researchers in public and private sectors.  Because this is a shared facility and investigators are dependent on this resource to complete their work, all users must follow the stated guidelines.  Failure to comply could result in denying access.

All perspective users must first meet with Doug Cromey, 626-2824,  cromey@arizona.edu.

All users are required to complete a training program before they can access the microscope on their own. This 3-part, 9 hour training will consist of a theoretical section, and two practical sessions. Users will receive a CD containing a copy of the facility policy, the user manual, and other resources.


All users of the facility are required to have successfully completed a LASER RADIATION PROTECTION COURSE, given by the University of Arizona Radiation Control Office. That office oversees Federal and Arizona State regulations for the proper use and handling of instruments that generate radiation. A "Radiation Worker Data Sheet" (RC-088), must be completed, signed by Doug Cromey and submitted to Radiation Control Office 10 days before the start of the class.  Bring a copy of the Certificate of Completion of the course to be filed in the Confocal lab.

Schedule of classes: See Radiation Control Office Schedule
Required form: RC-88

The Laser Safety Guide must be studied prior to attending the class. You may sign-out a copy through Doug Cromey.

All instructions for Start-up and Shut-down must be followed to insure that the condition of the microscope stays in good working order and that the lab is clean and organized for each user.

The LSM 510 is considered a laser hazard class IV instrument because of the potential of being exposed to the beam path of the Coherent MIRA 900 Ti-Sa 2-photon laser.  The dangerous laser radiation area of this laser includes the beam path and any secondary beam or reflected beam up to several meters.  Be careful not to expose yourself to the radiation of such lasers. The dangerous laser radiation area for the three visible light lasers is limited to the beam path and to a distance of up to around 10 cm from the specimen. You should always observe the following warnings:

  • * Do not place any reflecting objects in the beam path.
  • * Never open any covers or panels.
  • * Never look into the laser beam, not even with your remaining eye.
  • * Do not leave any objective positions of the nosepiece uncovered.
  • * Do not look at the specimen on the microscope during scanning.
  • * Eye protection (available in the room) must be worn while using the Ti-Sa 2-photon laser.
  • * Eye protection (available in the room) must be worn while using the Ti-Sa 2-photon laser.

Immediately report ANY exposure or accident to Doug Cromey, and to your Lab Supervisor. Apply appropriate First Aid techniques, and submit an accident form within 24 hours to Risk Management through Arizona Research Labs business office.

image Molecular Probes FluoCells2 prepared microscope slide

Links